![]() The levels of oxidized phospholipids in vivo is a critical question, as there is now substantial evidence that many of these compounds are bioactive and could contribute to pathology. A number of studies have investigated whether individual oxidized phospholipid products occur in vivo, and mass spectrometry techniques have been instrumental in detecting a variety of oxidation products in biological samples such as atherosclerotic plaque material, brain tissue, intestinal tissue and plasma, although relatively few have achieved an absolute quantitative analysis. Tandem mass spectrometry has proved invaluable in this respect and is generally the method of choice for structural work. Key to understanding the mechanisms of oxidation is the development of advanced and sensitive technologies that enable structural elucidation. Phospholipids can also be attacked by reactive nitrogen and chlorine species, further expanding the range of products to nitrated and chlorinated phospholipids. Common products are phospholipids with esterified isoprostane-like structures and chain-shortened products containing hydroxy, carbonyl or carboxylic acid groups the carbonyl-containing compounds are reactive and readily form adducts with proteins and other biomolecules. The number of end products that are formed following the initiation of phospholipid peroxidation is large, and is continually growing as new structures of oxidized phospholipids are elucidated. The oxidation of lipids has long been a topic of interest in biological and food sciences, and the fundamental principles of non-enzymatic free radical attack on phospholipids are well established, although questions about detail of the mechanisms remain. Vol 8, No 1 (2006): Serie D - Articles Place des alanine-aminotransferases dans la securite transfusionnelle : Etude au centre national de transfusion sanguine de Lome .Etude de la densite erythrocytaire par la methode des grandients de phtalate chez les patients drepanocytaires au Chu-campus de Lome(Togo) Abstract  ![]()
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